3D spheroids are a valuable tool for investigating the interplay between hypoxia and radioresistance. However, standardized methods for visualizing normoxic and hypoxic regions within spheroids and assessing treatment responses are lacking. We developed a straightforward workflow for the analysis of spheroids based on image processing using a novel custom-written script, and flow cytometry.
Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research.
缺氧和放射治疗相关研究中三维球体的活细胞成像和分析
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作者：Beckers Claire, Vasilikos Lazaros, Moor Lorena, Pruschy Martin
| 期刊： | Clinical and Translational Radiation Oncology | 影响因子： | 2.700 |
| 时间： | 2025 | 起止号： | 2025 Jan 15; 51:100920 |
| doi： | 10.1016/j.ctro.2025.100920 | 研究方向： | 细胞生物学 |
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