A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.
Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.
用于定量和可视化植物中谷氨酰胺的细菌全细胞生物传感器
阅读:11
作者:Tessaro Michael J, Soliman Sameh S M, Raizada Manish N
| 期刊: | Applied and Environmental Microbiology | 影响因子: | 3.700 |
| 时间: | 2012 | 起止号: | 2012 Jan;78(2):604-6 |
| doi: | 10.1128/AEM.05261-11 | 研究方向: | 细胞生物学 |
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