Bacterial whole-cell biosensor for glutamine with applications for quantifying and visualizing glutamine in plants.

用于定量和可视化植物中谷氨酰胺的细菌全细胞生物传感器

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作者:Tessaro Michael J, Soliman Sameh S M, Raizada Manish N
A whole-cell biosensor for glutamine (GlnLux) was constructed by transforming an Escherichia coli glutamine (Gln) auxotroph with a constitutive lux reporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman's r = 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.

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