Upcycling of Enzymatically Recovered Amino Acids from Textile Waste Blends: Approaches for Production of Valuable Second-Generation Bioproducts.

Tremendous quantities of textile waste generated and primarily landfilled annually represent a huge risk of contaminating the environment, together with loss of valuable resources. Especially, blended fabrics further pose a challenge for recycling and valorization strategies, while enzymatic hydrolysis offers a highly specific and environmentally friendly solution. In this study, we demonstrate that proteases specifically hydrolyze the wool components in blends with polyester, allowing recovery of pure polyester fibers as well as amino acids and peptides as platform molecules for further valorization. Recovered amino acids and peptides were successfully used as a nitrogen source for cultivation of Chlorella vulgaris and Rhodotorula mucilaginosa for the production of valuable biomolecules including pigments and lipids. Here, 11.3 mg/g(CDW) chlorophyll and 47% lipid content were obtained from algal biomass, while 1.1 mg/g(CDW) carotenoids and 35% lipids content were reached from the yeast grown on wool hydrolysate as the sole nitrogen source. These could be applied as natural dyes for textile applications or as biofuels to replace toxic synthetic compounds and fossil resources, respectively. The presented concept demonstrates feasibility of enzymatic recovery and microbial valorization of components of blended textile waste to support the development toward a circular bioeconomy.