Spectrophotometric and Multivariate Calibration Techniques for Simultaneous Determination of Different Drugs in Pharmaceutical Formulations and Human Urine: Evaluation of Greenness Profile.

Eco-friendly, rapid, and cost-effective two spectrophotometric methods were developed and validated for the determination of atenolol, paracetamol, hydrochlorothiazide, and levofloxacin. The first method is the newly developed extended derivative ratio (EDR) and the second method is multivariate curve resolution-alternating least squares (MCR-ALS). In the EDR method, the extended derivative ratio amplitudes at 281.6, 237.6, 279.2, and 282.8 nm were used for quantification of atenolol, paracetamol, hydrochlorothiazide, and levofloxacin, respectively. In the MCR-ALS method, calibration model was developed and correlation constraint was employed. External validation data set composed of seven mixtures was used, and different figures of merits such as root mean square error of prediction, standard error of prediction, bias, and relative error of prediction were calculated, and satisfactory results were obtained. Both methods provided comparable results. The methods were validated and applied for the determination of the target analytes in dosage forms, spiked and real human urine. Thereafter, the obtained results were statistically compared to the published methods and revealed no significant difference regarding accuracy and precision. Furthermore, the greenness profile of the methods was evaluated using the National Environmental Methods Index "NEMI" and Analytical Eco-Scale. The developed methods can be used as a valid eco-friendly and simple cost-effective alternative to the commonly used chromatographic methods for the routine analysis of the studied drugs in dosage forms and human urine.