Studies on cellulase-ultrasonic assisted extraction technology for flavonoids from Illicium verum residues.

BACKGROUND: Illicium verum is widely cultivated in southern China especially in Guangxi province. Its fruits has been traditionally used in Chinese medicine. In recent years, it has been the industrial source of shikimic acid. Usually the residues after extracting shikimic acid are treated as waste. Thus, the aim of this study was to optimize the extraction conditions of cellulase-ultrasonic assisted extraction technology for flavonoids from I. verum residues. RESULTS: The optimum extraction conditions with a maximum flavonoids yield of 14.76 % are as follows: the concentration of ethanol is 51.14 %, the liquid-solid ratio is 20.52 mL/g, the enzymatic hydrolysis pH is 5.303, the sonication time is 60 min, the enzyme solution temperature is kept at 45 °C, the amount of added enzyme is 70 mg/g, the enzymatic hydrolysis time is 2 h and the crushed mesh size is 0.355-0.85 mm. CONCLUSIONS: The data indicate that the cellulase-ultrasonic assisted extraction technology has the potential be used for the industrial production of flavonoids from I. verum.