The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Ã resolution.
Oil-free hyaluronic acid matrix for serial femtosecond crystallography.
用于连续飞秒晶体学的无油透明质酸基质
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| 期刊： | Scientific Reports | 影响因子： | 3.900 |
| 时间： | 2016 | 起止号： | 2016 Apr 18; 6:24484 |
| doi： | 10.1038/srep24484 | ||
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