The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Ã resolution.
Oil-free hyaluronic acid matrix for serial femtosecond crystallography.
用于连续飞秒晶体学的无油透明质酸基质
阅读：4
作者：Sugahara Michihiro, Song Changyong, Suzuki Mamoru, Masuda Tetsuya, Inoue Shigeyuki, Nakane Takanori, Yumoto Fumiaki, Nango Eriko, Tanaka Rie, Tono Kensuke, Joti Yasumasa, Kameshima Takashi, Hatsui Takaki, Yabashi Makina, Nureki Osamu, Numata Keiji, Iwata So
| 期刊： | Scientific Reports | 影响因子： | 3.900 |
| 时间： | 2016 | 起止号： | 2016 Apr 18; 6:24484 |
| doi： | 10.1038/srep24484 | ||
特别声明
1、本页面内容包含部分的内容是基于公开信息的合理引用；引用内容仅为补充信息，不代表本站立场。
2、若认为本页面引用内容涉及侵权，请及时与本站联系，我们将第一时间处理。
3、其他媒体/个人如需使用本页面原创内容，需注明“来源：[生知库]”并获得授权；使用引用内容的，需自行联系原作者获得许可。
4、投稿及合作请联系：info@biocloudy.com。