Abstract
Safe and effective methods for oral bacterial disinfection have been desired, since bacteria cause many infectious diseases such as dental caries, periodontal disease, and endodontic infections. Singlet oxygen ((1)O(2)) is attractive, because it is toxic to prokaryotic cells, but not to eukaryotic cells. We selected irradiation of titanium dioxide (TiO(2)) as a source of (1)O(2), because it has been used in sunscreens and cosmetic products without complications. In order to establish the optimal oral photodynamic therapy conditions, we measured the rate of (1)O(2) formation from the irradiated anatase or rutile forms of TiO(2) using 365 or 405 nm lamps. The rate of (1)O(2) formation decreased in the following order: anatase, 365 nm > rutile, 405 nm > rutile, 365 nm > anatase, 405 nm. Therefore, we concluded that irradiation of the rutile form of TiO(2) by a 405 nm lamp is the most favorable photodynamic therapy condition, because visible light is more desirable than UV light from the viewpoint of patient safety. We also confirmed that there was no direct HO(•) formation from the irradiated TiO(2).