Abstract
Rust fungi associated with Rubus were collected across diverse locations in Guizhou Province, and three new species - Gerwasiaamphidasydis on Rubusamphidasys, Phragmidiumcoreanicola on Rubuscoreanus, and Phragmidiumparvifolius on Rubusparvifolius are introduced. These novel species are described based on morphological characteristics and phylogenetic analysis of the ITS and LSU loci. Additionally, Gerwasiarubi-setchuenensis is introduced as a new host record on Rubusbuergeri. The study includes comprehensive morpho-anatomical descriptions, detailed illustrations, and a phylogenetic tree, providing insights into the taxonomic placement and relationships of these novel taxa within their respective lineages.