Abstract
Porphyrins, known as the "pigments of life", have evolved from their natural roles into versatile tools for biomedical applications. The development of activatable porphyrins has significantly expanded their utility, enabling precise responses to a carefully selected target analyte. These advances have broadened their use in imaging, diagnosis, and therapy. These capabilities are driven by activity-based sensing (ABS), which enhances the selectivity and sensitivity to various disease biomarkers. However, their design and implementation are intrinsically complex. This perspective provides an easy-to-follow roadmap that details how such molecules can be developed. Furthermore, we highlight recent progress in ABS-modified porphyrins, focusing on how specific modifications achieve these remarkable properties across various biomedical platforms. The ongoing evolution of activatable porphyrins holds great promise for the development of sophisticated, responsive systems, offering more effective diagnostic and therapeutic tools.