Abstract
In nanoscience, the "green" synthesis approach has received great interest as an eco-friendly and sustainable method for the fabrication of a wide array of nanoparticles. The present study accounts for an expeditious technique for the synthesis of silver nanoparticles (AgNPs) utilizing fruit waste grape pomace extracted tannin. Grape pomace tannin (Ta) involved in the reduction and capping of AgNPs and leads to the formation of stable Ta-AgNPs. Various conditions were attempted to optimize the particle size and morphology of Ta-AgNPs which was further analyzed using various analytical tools for different characteristic motives. UV-visible spectroscopy showed a characteristic peak at 420 nm, indicating successful synthesis of AgNPs. Energy disperses spectroscopy (EDS) analysis proved the purity of the produced Ta-AgNPs and manifested a strong signal at -2.98 keV, while Fourier-transform infrared spectrophotometer (FTIR) spectra of the Ta-AgNPs displayed the existence of functional groups of tannin. Zeta potential measurements (-28.48 mV) showed that the Ta-AgNPs have reasonably good stability. High resolution transmission electron microscopy (HR-TEM) analysis confirmed the average dimension of the synthesized NPs was estimated about 15-20 nm. Ta-AgNPs potentials were confirmed by in vitro antidiabetic activity to constrain carbohydrate digesting enzymes, mainly α-amylase and α-glucosidase, with a definite concentration of sample displaying 50% inhibition (IC(50)), which is about 43.94 and 48.5 μg/mL, respectively. Synthesized Ta-AgNPs exhibited significant antioxidant potential with respect to its 2,2'-azino-bis(3-ethylbenzothi-azoline-6-sulfonic acid) (ABTS) (IC(50) of 40.98 µg/mL) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC(50) of 53.98 µg/mL) free radical scavenging activities. Ta-AgNPs exhibited extraordinary antibacterial activity against selected pathogenic strains and showed comparable antimicrobial index against ampicillin as a positive control.