Abstract
In this study, the effects of Clove oil (CEO) on biofilm formation of Candida albicans (C. albicans) were investigated using XTT and crystal violet staining methods. The therapeutic efficacy of CEO was studied using ICR mouse model with candida vaginitis. The potential targets and pathways of the CEO's effect against C. albicans were predicted by network pharmacology. The relative gene levels of CDC42, Ste11, Hst7, CEK1, and CPH1 were evaluated by qPCR. Subsequently, the protein levels of CDC42, CEK1, and p-CEK1 were determined by Western blot. The results indicated CEO can inhibit C. albicans adhesion, mycelium formation and biofilm maturation process. By regulating the related genes and proteins, the CEO can inhibit C. albicans biofilm maturation process, and weaken the ability of C. albicans to cause disease. For candida vaginitis ICR mouse model, CEO can lower inflammatory cytokines levels, alleviated the symptoms of vaginitis, and play a good therapeutic effect.