Field detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR
定量PCR现场检测环境水样中的日本血吸虫尾蚴
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作者：Caitlin Worrell, Ning Xiao, Jorge E Vidal, Lin Chen, Bo Zhong, Justin Remais
Abstract
A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.
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