Abstract
The present study aimed to investigate the associations of tumor inhibitor of growth 4 (ING4) with tumor cell proliferation and microvessel density (MVD) in human glioma. Semi-quantitative reverse transcription polymerase chain reaction, western blotting and immunohistochemistry (IHC) were used to detect the level of ING4 expression in normal brain tissues and glioma tissues of different pathological grades. The cell proliferation index (PI) and the level of MVD were detected using IHC, facilitating the analysis of the correlation between ING4 and cellular proliferation and MVD in human glioma. The expression levels of ING4 mRNA and protein were significantly lower in the glioma tissues compared with the normal brain tissues (P<0.05) and decreased as the pathological grade of the glioma tissue increased (P<0.05). PI was reduced in the ING4 protein positively expressed (ING4+) glioma tissues compared with the ING4 protein negatively expressed (ING4-) glioma tissues (P≤0.01). The level of MVD was significantly higher in the glioma tissues than that in the normal brain tissues (P≤0.01), while the level of MVD decreased in the ING4+ glioma tissue compared with the ING4- glioma tissue (P≤0.001). A Spearman's rank correlation test revealed that ING4 protein expression was negatively correlated with PI and MVD. ING4 might inhibit tumor proliferation and angiogenesis in human glioma. The expression of ING4 was also associated with the pathological grading of the glioma tissue and negatively correlated with cellular proliferation and MVD in human glioma.