Abstract
Here, we describe a novel method for fabrication of microfluidic structures in classroom environments. This method is based on replica moulding of pasta structures in polydimethylsiloxane. Placing pasta structures on a petroleum jelly base layer enables templating round-shaped structures with controllable cross-sectional profiles. The pasta structures can be easily deformed and combined to create more complex 3D microfluidic structures. Proof-of-concept experiments indicate the capability of this method for studying the mixing of neighbouring flows, generation of droplets, lateral migration of particles, as well as culturing, shear stress stimulation, and imaging of cells. Our "do-it-in-classroom" method bridges the gap between the classroom and the laboratory.