Preparation of [32P]phosphatidylcholine and [32P]lysophosphatidylcholine by using soya beans

利用大豆制备[32P]磷脂酰胆碱和[32P]溶血磷脂酰胆碱

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Abstract

This method describes a procedure that can be carried out easily to obtain large amounts of [32P]phosphatidylcholine and [32P]lysophosphatidylcholine. The method involves germinating soya beans in the presence of [32P]Pi. The yield was 0.58% for [P]phosphatidylcholine and 0.52% for [32P]lysophosphatidylcholine, and the specific radioactivity of both was 10(7) d.p.m./mumol.

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