Abstract
Currently, human trophoblast stem cell (hTSC) is considered to be the most promising laboratory model stimulating trophoblast criteria. Our group has established hTSCs allowing differentiation to syncytiotrophoblast (STB) and extravillous trophoblast (EVT). Further, hTSC-based three-dimensional (3D) trophoblast organoid (hTSC-organoid) provides a transformative model for studying human placental development and the interaction between trophoblast and maternal environment. Here, we present a protocol to obtain different types of placental trophoblast cells and trophoblast organoids using hTSCs. The generation of hTSC-organoids takes 6 days. hTSC-organoids permit passaging and can differentiate into EVT lineage.