Abstract
OBJECTIVE: To determine the frequency of Factor V Leiden in cases of Deep Vein Thrombosis and Healthy controls. METHODS: This case control study was performed in Armed Forces Institute of Pathology Rawalpindi, From 21(st) March to 25(th) September 2013. One hundred patients with diagnostic evidence of Deep vein thrombosis on Doppler ultrasound/Magnetic resonance imaging (MRI) scan were included in the study through non probability convenient sampling and compared with 100 matched healthy controls. DNA was extracted from the blood sample by kit method. In order to identify Factor V Leiden mutation, the polymerase chain reaction (PCR) method was utilized combined with the Amplification refractory mutation system. Data was analyzed using statistical package for social sciences (SPSS) version 17. RESULTS: In 100 patients of Deep Vein Thrombosis (DVT), frequency of Factor V Leiden (FVL) was 13% and it is was 2% in healthy control group. A significant association was found between FVL and DVT with odds ratio of 7.32 and with P value (P = 0.003). CONCLUSION: FVL was found to be highly prevalent among patients of DVT, Signifying strong association between the two.