Analytical study of effective biodegradation of p-cresol using Serratia marcescens ABHI001: application in bioremediation

使用粘质沙雷氏菌 ABHI001 对对甲酚进行有效生物降解的分析研究:在生物修复中的应用

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作者:Tripti Singh, Neha Srivastava, A K Bhatiya, P K Mishra

Abstract

This study evaluated the capability of Serratia marcescens ABHI001 to effectively degrade p-cresol through different techniques. The molecular identity of the laboratory isolate S. marcescens ABHI001 was confirmed through the 16S ribosomal DNA gene pattern, and its morphological features were investigated through field-emission scanning electron microscopy. In addition, the degradation behavior of the isolate for cresol was verified using several techniques, including UV-visible spectroscopy, followed by high-performance liquid chromatography (HPLC), gas chromatography, and Fourier transform infrared spectroscopy. The maximum degradation percentage of 85% for p-cresol could be achieved after 18 h of incubation with S. marcescens ABHI001. The formation of p-hydroxybenzaldehyde, p-hydroxybenzoate, and protocatechuate metabolites was confirmed through HPLC. The study results indicate that S. marcescens ABHI001 may have applications in the bioremediation of organic pollutants, such as p-cresol.

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