Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques

使用分子技术鉴定沙特阿拉伯吉达的绵羊和山羊中的泰勒虫属

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作者:Dina M Metwally, Reem Alajmi, Muslimah N Alsulami, Isra M Al-Turaiki, Rewaida Abdel-Gaber, Afrah F Alkhuriji, Haleema H Albohiri, Khalil Mohamed, Hanadi B Baghdadi, Manal F El-Khadragy, Guillermo T Isaias, Saeed El-Ashram

Background

Thileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications.

Methods

Blood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Theileria spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of 18S rRNA followed by sequencing.

Results

According to obtained findings, Theileria spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that T. ovis identified in this study were found to be closely connected to an isolate from Turkey, with 84.4-99.8% pairwise identity and 52.35-99.79% coverage.

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