Detection of Candida albicans mRNA in archival histopathology samples by reverse transcription-PCR

利用逆转录-PCR技术检测存档组织病理样本中的白色念珠菌mRNA

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Abstract

The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age.

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