Abstract
This paper conducts an in-depth investigation and analysis of 25 microbiology course outlines from 23 domestic universities in China, focusing on the structure of prerequisite courses. The study finds that microbiology course outlines typically include basic course information, objectives, content, teaching methods, resources, assessment, and scheduling. Reasonable prerequisite course settings are vital for clarifying logical relationships among courses in talent training programs, organizing key and challenging knowledge systems, and enhancing university course quality. The paper proposes recommendations for microbiology prerequisite course design, including identifying core prerequisites, ensuring logical sequence rationality, setting appropriate numbers of prerequisites, and considering professional specificities. These suggestions offer a reference for optimizing microbiology course outline design and improving biological talent cultivation quality. The paper also explores the selection frequency and number of prerequisite courses. The top three selected courses are biochemistry, general biology, and genetics, while the least selected are biostatistics, physiology, and ecology. It is recommended that universities carefully consider course logical sequences and students' actual learning needs when setting microbiology prerequisite courses to ensure students build a solid knowledge foundation and smoothly transition to microbiology learning.