Inhibitory effect of Pseudomonas aeruginosa on the phagacytic and killing activities of rabbit polymorphonuclear leukocytes: purification and characterization of an inhibitor of polymorphonuclear leukocyte function

A clinically isolated strain of polymorphonuclear leukocyte (PMN)-resistant Pseudomonas was found to produce an extracellular substance (PMN inhibitor) that inhibits the phagocytic and killing activities of PMN. The PMN inhibitor was purified from culture filtrates by precipitation with (NH4)2SO4 and chromatography on phosphocellulose, diethylaminoethyl-cellulose, and Sephadex G-100. The preparation was homogenous as judged by disc gel electrophoresis. The purified PMN inhibitor appeared to be a protein with a molecular weight of approximately 65,000 that was inactivated by heating and by exposure to a proteolytic enzyme.