High-throughput sequencing revealed low-efficacy genome editing using Cas9 RNPs electroporation and single-celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis

高通量测序揭示了使用Cas9 RNP进行基因组编辑的效率较低，电穿孔和单细胞显微注射为将CRISPR试剂递送到眼虫中提供了一种替代方法。