Abstract
The genus Neobythites is the most diverse group in the family Ophidiidae. In this study, we assembled and reported the complete mitochondrial genome of N. sivicola for the first time. The mitochondrial genome is 17,316 bp in length and contains 13 PCGs, 2 rRNAs, and 22 tRNAs. Phylogenetic analysis revealed that N. sivicola was the sister species to N. unimaculatus, and Neobythites was closer to Sirembo. This study significantly contributes to the genomic resources available for the family Ophidiidae and the genus Neobythites, providing a valuable molecular resource for further research on the taxonomy and phylogenetics of this family.