Abstract
BACKGROUND: Pancorius Simon, 1902 is a relatively large genus of jumping spider family Salticidae and currently contains 42 valid species that are mainly distributed in South East Asia, 11 of which are recorded from China. NEW INFORMATION: A new spider species of the genus Pancorius from Guiyang City in southwest China, is described under the name of P.guiyang Yang, Gu & Yu, sp. nov. Detailed descriptions and photographs are provided. DNA barcodes (a partial fragment of the mitochondrial cytochrome oxidase subunit I gene, COI) of the species were obtained to confirm matching of the sexes and for future use in molecular studies.