Advanced glycation end products alter steroidogenic gene expression by granulosa cells: an effect partially reversible by vitamin D

晚期糖基化终产物改变颗粒细胞的类固醇生成基因表达:维生素 D 可部分逆转这一影响

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作者:Z Merhi, E Buyuk, M J Cipolla

Methods

Follicular fluid levels of the anti-inflammatory soluble RAGE (sRAGE), AGEs and 25-hydroxyvitamin D (25-OHD) were quantified for possible correlations. GCs of each participant were split equally and treated with either media alone (control) or with human glycated albumin (HGA as a precursor for AGEs) with or without vit D3 after which RT-PCR and immunofluorescence were performed and cell culture media estradiol (E2) levels were compared. Main

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