Expression, purification, crystallization and preliminary X-ray analysis of NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver

甲状腺切除鸡脂肪肝中特异性表达的 NAD(P)H 依赖性羰基还原酶的表达、纯化、结晶和初步 X 射线分析

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作者:Kazunari Yoneda, Yudai Fukuda, Takeshi Shibata, Tomohiro Araki, Takahiro Nikki, Haruhiko Sakuraba, Toshihisa Ohshima

Abstract

An NAD(P)H-dependent carbonyl reductase specifically expressed in thyroidectomized chicken fatty liver was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 300 as the precipitant. The crystals belonged to the monoclinic space group C2, with unit-cell parameters a=104.26, b=81.32, c=77.27 Å, β=119.43°, and diffracted to 1.86 Å resolution on beamline NE3A at the Photon Factory. The overall Rmerge was 5.4% and the data completeness was 99.4%.

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