Abstract
OBJECTIVE: To investigate the effects of micro/nano-structure and antimicrobial peptides (AMPs) on antibacterial properties for titanium (Ti) metal surface. METHODS: Ti disks were treated via sandblasted large-grit acid-etched (SLA) and alkali-heat treatment (AHT) to build the micor/nano-structure, on which AMPs were spin-coated with a certain amount (10, 30, 50, 70, and 90 μg). Scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) were used to observe the surface structure and characterize the surface elements (i.e. contents of C, N, O, and Ti). Ti disks loaded with AMPs of difference amounts were co-cultured with Staphylococcus aureus ( S. aureus) for 24 hours. After that, the formation and dimension of antibacterial circle were measured. Furthermore, the Ti disks treated with different approaches (untreated, SLA treatment, SLA+THA treatment, and loaded with 90 μg AMPs) were co-cultured with S. aureus and Escherichia coli ( E.coli) for 3 hours, bacterial adhesion on the disks were evaluated by using SEM. The antibacterial performances in solution were quantitatively evaluated by immersing the Ti disks in bacterial solutions and measuring the absorbance ( A) values. RESULTS: It was found that the nanoporous structure could be easily constructed by SLA+AHT approach. After spin-coating AMPs, the nanopores with the diameter less than 200 nm were almost covered. According to the element analysis, with the increase of AMPs, the C content gradually increased; the N content was not detected until AMPs amount reached 70 μg on the disks. The diameter of antibacterial circle clearly depended on the AMPs amount. The Ti disks loaded with 90 μg AMPs had significantly larger antibacterial circles than the other Ti disks ( P<0.05). Based on the SEM observation, the Ti disks loaded with 90 μg AMPs has the least bacterial attachment compared with the other Ti disks ( P<0.05). The A value of bacterial solution immersed with the Ti disks loaded with 90 μg AMPs was much lower than the other Ti disks ( P<0.05). CONCLUSION: The approach of micro/nano-structure and AMPs can improve the antibacterial properties of Ti metal surface.