Abstract
BACKGROUND AND AIMS: Thyroid cancer, a prevalent endocrine malignancy, often presents as thyroid nodules, whose benign or malignant nature is challenging to determine. This study aims to identify circulating miRNA panels that may distinguish between benign nodules, papillary thyroid cancer, and normal thyroid conditions, building on extensive research into miRNAs as potential thyroid cancer biomarkers. MATERIALS AND METHODS: As a cross-sectional case-control study the study revealed the quantification of the 17-miRNA panel was evaluated using qRT-PCR method on 60 blood samples, comprising 25 patients diagnosed with PTC, 24 patients with benign lesions, and 11 healthy controls. RESULTS: We found that seven miRNAs, including miR144-3p and miR190a-5p, exhibited statistically significant variations in their expression levels, and miR144-3p exhibited the most superior diagnostic capability in distinguishing between malignant and healthy groups, as well as between benign and healthy groups. The concurrent utilization of miR144-3p, miR148a-3p, and miR190a-5p resulted in an enhancement of sensitivity from 76% to 88%. CONCLUSION: Our study suggests that miR144-3p and miR190a-5p exhibit potential as biomarkers for distinguishing between benign and malignant thyroid nodules and healthy individuals, and further investigation is necessary to evaluate their clinical significance.