Conclusions
Evaporation using freeze drying/centrifugal concentration is an automated process, reducing overall processing time for analysis of numerous samples.
Methods
Samples were evaporated using freeze drying, centrifugal concentration, and nitrogen flow and analyzed by LC-MS/MS.
Objective
Compare evaporation techniques in the work-up procedure of saliva samples for cortisol analysis by a previously validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Design and
Results
Cortisol concentrations detected using each technique did not significantly differ. Factors other than the recovery of cortisol were evaluated. Conclusions: Evaporation using freeze drying/centrifugal concentration is an automated process, reducing overall processing time for analysis of numerous samples.