GeoChip profiling of microbial community in response to global changes simulated by soil transplant and cropping

利用GeoChip技术分析土壤移植和耕作模拟全球变化下微生物群落的变化

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Abstract

Microbe plays an important role in driving biogeochemical cycles, thus it is of great interest to understand microbial responses and feedbacks to global changes. We have recently analyzed functional potentials of soil microbial community via a high-throughput, microarray-based metagenomic tool named GeoChip 3.0 to illustrate microbial responses to global changes simulated by soil transplant and/or maize cropping. Here we describe detailed experimental design, data collection and pre-processing to support our published studies by Liu et al. [5] and Zhao et al. [14].

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