Background
Colon cancer is the most frequently lethal cancer in digestive system. Herein, we tested the influences of UNC5B antisense lncRNA 1 (UNC5B-AS1) on colon cancer cell growth and metastasis, along with the regulatory function of UNC5B-AS1 in microRNA-622 (miR-622) expression.
Conclusion
Silencing UNC5B-AS1 repressed colon cancer growth and metastasis might be through raising miR-622 expression and suppressing AMPK and PI3K/AKT pathways.
Methods
Firstly, UNC5B-AS1 expression in colon cancer tissues and corresponding normal tissues were tested. Then, the influences of silencing UNC5B-AS1 by sh-UNC5B-AS1 transfection on colon cancer HCT116 and Caco-2 cell viability, proliferation, migration, invasion and apoptosis, as well as miR-622 expression were assessed, respectively. Subsequently, whether miR-622 attended to the influences of silencing UNC5B-AS1 on HCT116 and Caco-2 cells were probed. Finally, the activities of AMPK and PI3K/AKT pathways in cells were analysed.
Results
UNC5B-AS1 had high expression level in colon cancer tissues. Silencing UNC5B-AS1 repressed HCT116 and Caco-2 cell proliferation, migration and invasion, but boosted cell apoptosis. Moreover, silencing UNC5B-AS1 raised miR-622 expression in HCT116 and Caco-2 cells. miR-622 inhibitor transfection weakened the influences of silencing UNC5B-AS1 on HCT116 and Caco-2 cells. Besides, Silencing UNC5B-AS1 suppressed AMPK and PI3K/AKT pathways via raising miR-622.