Abstract
We used gas chromatography-mass spectrometry (GC-MS) with an untargeted metabolomics approach to look at the metabolite profiles of traditional Iranian yogurts made from cow, goat, buffalo, and sheep milk. Results showed that different animal milks significantly influenced physicochemical properties and fatty acid (FA) composition, resulting in diverse metabolites. Over 80 % of all the fatty acids in the yogurt samples were saturated. The main fatty acids found were myristic acid (C14:0), palmitic acid (C16:0), and oleic acid + petroselenic acid (cis-9 C18:1 + cis-6 C18:1). In total, 36 metabolites, including esters, aldehydes, alcohols, and acids, were detected. Some important metabolites that changed yogurt profiles were 2-heptanone, methyl acetate, 2-propanone, butyl formate, and 4-methyl benzal. Associations between metabolite profiles and milk compositional traits were also observed, with statistical models showing a strong correlation between metabolite profiles and FA content. This study is the first to explore the impact of different animal sources and regions in Iran on the metabolome profiles of traditional yogurts. These results give us useful information about how metabolites differ between species and can be used to make new dairy products based on milk compositions and metabolites, which will help with future formulations of autochthonous starters.