Abstract
The collection of environmental DNA (eDNA) is a relatively new, non-invasive and effective method for detecting the presence of rare or endangered species, invasive alien species, and monitoring fish and wildlife populations, thus contributing to better conservation of natural environments. Academic researchers are its main users. The reasons for its slow diffusion among other potential users remain poorly documented to date. This study aimed to characterize the barriers and levers to the adoption of eDNA by distinct types of end-users, depending on the contexts in which they operate. We conducted semi-structured interviews with 33 participants to document and analyze their perceptions of eDNA. The Unified Theory of Acceptance and Use of Technology (UTAUT) inspired our analysis. Our findings revealed that potential end-users perceive the eDNA-based methods positively, although they are improvable. A lack of knowledge about its limitations and potential affects how useful eDNA is perceived to be and potential end-users' confidence in its results. We propose action levers to increase potential end-users' trust in the method and its compatibility with their current practices, and identify avenues to facilitate its diffusion.