Fertilization rate and number of embryos on day 2 after intrauterine and deep intrauterine insemination using frozen-thawed boar semen in multiparous sows

使用冷冻解冻公猪精液对经产母猪进行宫内和深部宫内人工授精后第2天的受精率和胚胎数量

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Abstract

The present study determines fertilization rate and number of embryos on Day 2 after intrauterine insemination (IUI) and deep intrauterine insemination (DIUI) using frozen-thawed (FT) boar semen in multiparous sows. Twelve crossbred Landrace × Yorkshire multiparous sows were included. The sows were inseminated at 24 h after oestrus detection and reinseminated every 12 h until ovulation took place. The inseminations were conducted using IUI with 2 × 10(9) FT sperm per dose (n = 6) and DIUI with 1 × 10(9) FT sperm per dose (n = 6). The sows were slaughtered at 45.1 ± 7.2 h after ovulation. Embryos and unfertilized oocytes were flushed from the oviducts. IUI yielded a better fertilization rate than DIUI (66.0% versus 31.0%, P < .001). The number of embryos was 13.5 ± 2.7 and 6.6 ± 3.2 embryos/sow in IUI and DIUI groups, respectively (P = .08). The proportion of sows having unilateral fertilization was higher in the DIUI (3/5) than the IUI group (1/6). In conclusion, IUI with at least 2 × 10(9) total number of FT boar spermatozoa is recommended.

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