Abstract
Dissecting the basis of naturally occurring trait variation is one of the central goals of modern genetics. For eukaryotes, classic methods for this purpose rely on screens of recombinants from matings between distinct parents. These tools cannot be used in studies of species that cannot mate to form recombinant progeny in the first place. However, new approaches are coming online to shuffle the genomes of otherwise incompatible species. With them, geneticists can elucidate how evolution built a new trait, even if it happened millions of years ago, in a lineage that is now reproductively cutoff from its closest relatives.