Conclusion
We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.
Methods
Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.
Objective
To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies. Materials and
Results
Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.