Quantitative high-throughput population dynamics in continuous-culture by automated microscopy

利用自动化显微镜对连续培养中的高通量群体动力学进行定量分析

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Abstract

We present a high-throughput method to measure abundance dynamics in microbial communities sustained in continuous-culture. Our method uses custom epi-fluorescence microscopes to automatically image single cells drawn from a continuously-cultured population while precisely controlling culture conditions. For clonal populations of Escherichia coli our instrument reveals history-dependent resilience and growth rate dependent aggregation.

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