Real-time measurement of pyrophosphate release kinetics
焦磷酸释放动力学的实时测量
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作者：Jeremiah W Hanes, Kenneth A Johnson
Abstract
In order to measure the kinetics of pyrophosphate release, we have coupled pyrophosphatase to the fluorescently labeled phosphate binding protein developed by Webb. We show that the assay is capable of measuring rates of pyrophosphate release up to 100 s−1.
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