Intrinsic role of coagulase negative staphylococci norA-like efflux system in fluoroquinolones resistance

凝固酶阴性葡萄球菌norA样外排系统在氟喹诺酮类耐药性中的内在作用

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Abstract

NorA is a Staphylococcus aureus multidrug transporter that exports structurally distinct compounds including fluoroquinolones. In this study norA-like genes of Staphylococcus epidermidis (norA (SEP)) and Staphylococcus haemolyticus (norA (SHAE)) were identified and sequenced. The nucleotide identity of norA (SEP) and norA (SHAE) with norA was 75.3 and 74.1%, respectively, and the amino acid identity 87.7 and 86%, respectively. Inactivation of norA (SEP) increased the ciprofloxacin susceptibility of E. coli DH5α carrying the pB SK 198 norA (SEP) EZ cat norA (SEP) plasmid.

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