Abstract
Nasopharyngeal cancer (NPC) is a malignant tumor of the head and neck, which is extremely sensitive to radiotherapy. The aim of this study is to evaluate the feasibility of a label-free nanobiosensor based on plasma surface-enhanced Raman spectroscopy (SERS) to assess the radiotherapy effect in NPC. Here, SERS measurements were performed on plasma samples from 40 pre-treatment and post-treatment NPC as well as 30 healthy volunteers. Results demonstrate that the spectral characteristic of post-treatment samples is obviously different from that of pre-treatment ones, owing to the changes of biomolecules in plasma induced by radiotherapy. Classification sensitivities of 83.3%, 61.8% and 95.1%, and specificities of 91.2%, 67.4% and 93% can be achieved for separating pre- and post-treatment samples, post-treatment and normal samples, and pre-treatment and normal samples, respectively, suggesting the great potential of plasma SERS method as a rapid and convenient tool for radiotherapy assessment and cancer screening in NPC.