Abstract
Pseudonocardia autotrophica NBRC 12743 contains a cytochrome P450 vitamin D(3) hydroxylase, and it is used as a biocatalyst for the commercial production of hydroxyvitamin D(3), a valuable compound for medication. Here, we report the complete genome sequence of P. autotrophica NBRC 12743, which could be useful for improving the productivity of hydroxyvitamin D(3).