Abstract
BACKGROUND: Chinese Tibetan medicine plays a crucial role in complementary anti-tumor treatments. This article aims to investigate the inhibitory effect of the alcoholic extract of Tibetan medicine Deziyangxin (DZYX) on the proliferation and migration of non-small cell lung cancer (NSCLC) cells, specifically LLC cells, as well as explore its potential mechanism of action. METHODS: The effect of the alcoholic extract on LLC cell viability was assessed using the CCK-8 method. The proliferation of LLC cells was evaluated using the EdU (5-Acetyl-2'-deoxyuridine) assay. Transwell assays were conducted to measure cell metastasis. Western blot analysis was performed to assess the expression of Cleaved Caspase-3, Bcl-2, Beciln-1, indicating the impact of DZYX on apoptosis and autophagy in LLC cells. Furthermore, the anti-tumor mechanism of DZYX was explored through transcriptome research and detection of Akt, p-Akt, p-mTOR protein levels. RESULTS: The ethanol extract of DZYX exhibited a concentration-dependent and time-dependent inhibitory effect on LLC cell viability, with an IC50 of 406.1 μg/ml. Moreover, the ethanol extract of DZYX significantly reduced the migration ability of LLC cells. Additionally, the alcoholic extract of DZYX upregulated the expression of Cleaved Caspase-3 and Beciln-1 proteins, while downregulating the expression of Bcl-2 in LLC cells. Importantly, DZYX ethanol extract down regulated the expression of Akt and p-mTOR proteins in LLC cells, which combined with transcriptome results indicated that the drug exerted a multi-target and multi-pathway effect, primarily related to inhibiting the activation of the PI3K/AKT/m-TOR signaling pathway. CONCLUSION: The alcoholic extract of DZYX demonstrates inhibitory effects on LLC cells, promoting apoptosis and autophagy. It is hypothesized that its anti-tumor mechanism is associated with the PI3K/AKT/m-TOR pathway.