Ficus benjamina, the weeping fig, is one of the most widely distributed and cultivated figs, with important ecological functions and landscape value. However, the lack of a reference genome has hindered molecular and functional research on this well-known fig-tree. Here we present a chromosome-scale genome assembly and annotation for F. benjamina, based on a combination of Illumina short-reads, PacBio subreads, and Hi-C sequencing data. The genome consists of 13 pseudochromosomes that contain 362.73 Mb of assembled sequences, with a contig N50 length of 25.76 Mb and a complete BUSCO score of 98.10%. In total, 28,840 protein-coding genes were identified, of which 96.22% were functionally annotated. Our study provides the first chromosome-level genome of F. benjamina, providing an important resource for exploring the genetic basis of its ecological and horticultural characters.