This study investigates how different levels of extrusion pressure during 3D printing affect the cell viability of human bronchial epithelial (HBE) cells embedded in printed samples. In this study, samples were printed at three levels of extrusion pressure. The cell viability was assessed through live/dead staining via microscopic imaging. The results show that increasing the extrusion pressure from 50 to 100 kPa led to a higher degree of cell death. These results demonstrate how the extrusion pressure affects the viability of HBE cells and provide a basis for future studies on pressure-induced responses in respiratory tissues.