CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions

CRISPR 筛选可全面评估癌症的脆弱性，但会对高度扩增的基因组区域产生假阳性结果

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作者：Diana M Munoz, Pamela J Cassiani, Li Li, Eric Billy, Joshua M Korn, Michael D Jones, Javad Golji, David A Ruddy, Kristine Yu, Gregory McAllister, Antoine DeWeck, Dorothee Abramowski, Jessica Wan, Matthew D Shirley, Sarah Y Neshat, Daniel Rakiec, Rosalie de Beaumont, Odile Weber, Audrey Kauffmann, E
期刊：Cancer Discovery影响因子：29.700
时间：2016起止号：2016 Aug;6(8):900-13.
doi：10.1158/2159-8290.CD-16-0178研究方向： 肿瘤

Significance

We show in this study that CRISPR-based screens have a significantly lower false-negative rate compared with RNAi-based screens, but have specific liabilities particularly in the interrogation of regions of genome amplification. Therefore, this study provides critical insights for applying CRISPR-based screens toward the systematic identification of new cancer targets. Cancer Discov; 6(8); 900-13. ©2016 AACR.See related commentary by Sheel and Xue, p. 824See related article by Aguirre et al., p. 914This article is highlighted in the In This Issue feature, p. 803.

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