Discussion
Our data indicate that WJ-MSC-CM could be considered an appealing strategy to both topical and systemic administrations in SSc patients.
Methods
We analyzed the content of MSC-conditioned media (MSC-CM) obtained from MSCs isolated from adipose tissue (AT), bone marrow (BM), Wharton's jelly (WJ), and cord blood (CB) by ELISA method, and their effects on the wound healing process by fibroblast proliferation, migration, and ECM deposition assays, to compare regenerative potential of different MSC populations.
Results
WJ-MSC-conditioned medium (CM) and BM-MSC-CM show a greater regenerative profile, compared to CB-MSC-CM and AT-MSC-CM, due to the abundance of growth factors and immunomodulatory cytokines and the effects on fibroblast functions. In SSc fibroblasts, WJ-MSC-CM significantly promotes fibroblast-mediated wound healing processes and VEGF expression, compared to BM-MSC-CM.