Rapamycin enhances HBV production by inducing cellular autophagy

雷帕霉素通过诱导细胞自噬增强 HBV 的产生

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作者:Wenjuan Huang, Fengrong Zhao, Ying Huang, Xia Li, Sufei Zhu, Qin Hu, Weixian Chen

Background

Some reports revealed that rapamycin could reactivate HBV infection. However, the mechanism has not been clearly explained. Objectives: In this report, we studied the mechanism by which rapamycin enhances HBV replication and expression by inducing cellular autophagy. Materials and

Conclusions

These results indicated a potential explanation for reactivation of HBV infection when patients with hepatitis receive rapamycin.

Methods

HepG2.2.15 cells were treated with rapamycin to induce autophagy. Autophagosomes were observed by fluorescence microscopy and transmission electron microscopy. Autophagy marker protein LC3-Ⅱ/LC3-Ⅰwas detected by Western blotting. HBV DNA and mRNA were determined by real time PCR and Southern blotting. HBsAg was evaluated by ELISA.

Results

In HepG2.2.15 cells, HBV DNA and HBsAg increased when host cells were treated with rapamycin and the effect was reversed by autophagy inhibitor, 3-methyladenine (3-MA). Conclusions: These results indicated a potential explanation for reactivation of HBV infection when patients with hepatitis receive rapamycin.

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