Nature Protocols

In situ structure determination of conformationally flexible targets using nextPYP.

Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.

适用于冷冻电镜的微晶电子衍射样品的全面制备

A guide to building a low-cost centrifuge force microscope module for single-molecule force experiments.

构建低成本离心力显微镜模块用于单分子力实验的指南

Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells.

唾液酸适体和 RNA 原位杂交介导的邻近连接分析用于单细胞中糖 RNA 的空间成像

Droplet-based functional CRISPR screening of cell-cell interactions by SPEAC-seq.

基于液滴的 CRISPR 功能筛选细胞间相互作用的 SPEAC-seq 技术

High-confidence and high-throughput quantification of synapse engulfment by oligodendrocyte precursor cells.

高置信度和高通量定量分析少突胶质细胞前体细胞对突触的吞噬作用

Mass spectrometry-based top-down proteomics for proteoform profiling of protein coronas.

基于质谱的自上而下蛋白质组学方法用于蛋白质冠的蛋白质组学分析

Visually guided in vivo single-cell electroporation for monitoring and manipulating mammalian hippocampal neurons.

视觉引导的体内单细胞电穿孔技术用于监测和操控哺乳动物海马神经元

Sonication-assisted protein extraction improves proteomic detection of membrane-bound and DNA-binding proteins from tumor tissues.

超声辅助蛋白质提取可提高肿瘤组织中膜结合蛋白和 DNA 结合蛋白的蛋白质组学检测

Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers.

Orbitrap 分析仪上多重电荷检测质谱的标准化工作流程