Development of a Rapid and Efficient Protocol for Seed Germination and Seedling Establishment of Oryza coarctata.

Seed germination is a critical and challenging process in the propagation of Oryza coarctata, a wild halophytic rice species. This protocol outlines the seed germination procedure for O. coarctata. All steps required for optimal germination and seedling establishment of O. coarctata in both sterile and soil-based systems are described in detail. Additionally, the protocol includes an analysis of the primary hormones, abscisic acid (ABA) and gibberellin (GA), involved in regulating seed dormancy and germination. Key features • This protocol provides detailed instructions for germinating Oryza coarctata and improving its germination rate. • The protocol enables the successful vegetative and reproductive growth of O. coarctata in soil. • Additionally, the protocol outlines the analysis of hormones (abscisic acid and gibberellin) that play key roles in the germination process. Graphical overview Protocol for germination, seedling development, and hormone analysis of Oryza coarctata seeds. Step-by-step instructions include germinating seeds on filter papers or sterile growing medium; the established seedlings can be grown in controlled environments or a greenhouse. Extraction and analysis of endogenous key hormones offer insights into their roles in controlling dormancy and germination.